During the mid-1960s, the Deutsche Bundesbahn was looking for an inexpensive replacement for the steam locomotives from the 82 and 94.5 series complete with counter-pressure brakes that were suitable for the last steep routes. In doing so, the decision was made to equip 10 locomotives from the third and final batch of the V100.20 with an additional hydrodynamic brake alongside the compressed air brake and handbrake. The most striking distinguishing feature of the V100 2332 to 2341 compared to the V100.20 series is the large air intake grille on the right hand side of the long front end directly behind the cooler shutters as well as the omission of the auxiliary diesel unit.